Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-03 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 1 |
| Spacegroup name | P 32 |
| Unit cell lengths | 86.238, 86.238, 217.487 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.920 - 2.130 |
| R-factor | 0.221 |
| Rwork | 0.220 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.010 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.8 (20-APR-2021)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.690 | 2.270 |
| High resolution limit [Å] | 2.130 | 2.130 |
| Number of reflections | 445721 | 23235 |
| <I/σ(I)> | 10.8 | 1.5 |
| Completeness [%] | 94.4 | |
| Redundancy | 5.3 | |
| CC(1/2) | 0.998 | 0.544 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.2 M magnesium formate, 20% (w/v) PEG 3,350, 0.1 M magnesium chloride tetrathydrate |
