9MGL

Crystal structure of PRMT5:MEP50 in complex with MTA and GSK3326595

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-09
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97950
Spacegroup name	I 2 2 2
Unit cell lengths	99.740, 136.970, 178.890
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.620 - 2.250
R-factor	0.1957
Rwork	0.192
R-free	0.26770
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.246
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	108.755	108.750	2.580
High resolution limit [Å]	2.250	8.390	2.250
Rmerge	0.116	0.040	1.028
Rpim	0.049	0.018	0.456
Number of reflections	24898	7477	7505
<I/σ(I)>	11.3	34.1	1.8
Completeness [%]	94.3	99.8	81
Redundancy	6.5	6	6
CC(1/2)	0.998	0.999	0.668

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		283	35% PEG 3350, 327 mM ammonium sulfate