9MG4
Structure of Saccharomyces cerevisiae mRNA cap (guanine-N7) methyltransferase, Abd1, in complex with SAH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-10-14 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.98 |
Spacegroup name | H 3 2 |
Unit cell lengths | 95.160, 95.160, 248.942 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 24.830 - 1.750 |
R-factor | 0.1648 |
Rwork | 0.164 |
R-free | 0.18630 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.811 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.21rc1_5156)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.830 | 1.790 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.186 | 0.606 |
Rmeas | 0.194 | 0.637 |
Rpim | 0.057 | 0.196 |
Total number of observations | 494627 | 25862 |
Number of reflections | 43915 | 2478 |
<I/σ(I)> | 9 | 3.2 |
Completeness [%] | 100.0 | |
Redundancy | 11.3 | 10.4 |
CC(1/2) | 0.989 | 0.897 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 292 | 0.8 M Sodium Phosphate, 1.2 M Potassium Phosphate, and 0.1 M Sodium Acetate |