9MG4
Structure of Saccharomyces cerevisiae mRNA cap (guanine-N7) methyltransferase, Abd1, in complex with SAH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.98 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 95.160, 95.160, 248.942 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.830 - 1.750 |
| R-factor | 0.1648 |
| Rwork | 0.164 |
| R-free | 0.18630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.811 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.21rc1_5156)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.830 | 1.790 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.186 | 0.606 |
| Rmeas | 0.194 | 0.637 |
| Rpim | 0.057 | 0.196 |
| Total number of observations | 494627 | 25862 |
| Number of reflections | 43915 | 2478 |
| <I/σ(I)> | 9 | 3.2 |
| Completeness [%] | 100.0 | |
| Redundancy | 11.3 | 10.4 |
| CC(1/2) | 0.989 | 0.897 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 292 | 0.8 M Sodium Phosphate, 1.2 M Potassium Phosphate, and 0.1 M Sodium Acetate |
