9MG2
Structure of Kluyveromyces lactis mRNA cap (guanine-N7) methyltransferase, Abd1, in complex with sinefungin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.075 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.962, 54.789, 112.933 |
| Unit cell angles | 90.00, 92.80, 90.00 |
Refinement procedure
| Resolution | 24.640 - 1.590 |
| R-factor | 0.1884 |
| Rwork | 0.187 |
| R-free | 0.21870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.796 |
| Data reduction software | HKL-2000 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.21rc1_5156)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.620 |
| High resolution limit [Å] | 1.590 | 4.320 | 1.590 |
| Rmerge | 0.058 | 0.027 | 0.525 |
| Total number of observations | 143857 | ||
| Number of reflections | 67709 | 3875 | 3401 |
| <I/σ(I)> | 9.5 | ||
| Completeness [%] | 89.6 | 98.4 | 91.7 |
| Redundancy | 2.1 | 2.2 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 292 | 0.2 M Magesium Chloride, and 25% (w/v) PEG 3350 |
