9MG0
Structure of Kluyveromyces lactis mRNA cap (guanine-N7) methyltransferase, Abd1, in complex with SAH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.075 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.616, 64.821, 73.524 |
| Unit cell angles | 90.00, 94.48, 90.00 |
Refinement procedure
| Resolution | 24.960 - 1.300 |
| R-factor | 0.1665 |
| Rwork | 0.165 |
| R-free | 0.18670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.992 |
| Data reduction software | HKL-2000 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.21rc1_5156)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.320 |
| High resolution limit [Å] | 1.300 | 3.530 | 1.300 |
| Rmerge | 0.059 | 0.030 | 0.702 |
| Total number of observations | 576584 | ||
| Number of reflections | 143920 | 7073 | 7068 |
| <I/σ(I)> | 12.4 | ||
| Completeness [%] | 98.6 | 94.5 | 97.2 |
| Redundancy | 4 | 4 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 292 | 0.2 M Calcium Chloride, 20% (w/v) PEG 3350, and 0.1 M Potassium Sodium Tartrate |
