9MFP

Cat DHX9 in Complex with Compound 1 and ADP

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-06-02
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97918
Spacegroup name	P 43 21 2
Unit cell lengths	86.470, 86.470, 351.170
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.520 - 2.270
R-factor	0.19712
Rwork	0.195
R-free	0.24593
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.405
Data reduction software	Aimless
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.520	2.329
High resolution limit [Å]	2.270	2.270
Rmerge	0.123	1.616
Number of reflections	59824	4317
<I/σ(I)>	18.5	2.4
Completeness [%]	100.0	99.91
Redundancy	25.5	27.4
CC(1/2)	0.999	0.766

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.15 M Ammonium sulfate, 0.1 M TRIS pH 8, 15% w/v PEG 4000