9MEV

Structure of H1H3:FluA20 Chimeric Antigen Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-02-02
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	50.641, 93.465, 86.434
Unit cell angles	90.00, 93.48, 90.00

Refinement procedure

Resolution	44.820 - 2.060
R-factor	0.1821
Rwork	0.181
R-free	0.20910
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.290
Data reduction software	XDS
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.820	2.090
High resolution limit [Å]	2.060	2.060
Rmeas	0.067
Number of reflections	45825	945
<I/σ(I)>	17.9
Completeness [%]	92.1
Redundancy	3
CC(1/2)	0.987	0.811

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	0.1 M PCB, pH 6, 25% w/v PEG1500