9MER

Structure of H1H5:FluA20 Chimeric Influenza HA Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-02-02
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 43 21 2
Unit cell lengths	79.533, 79.533, 256.452
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.980 - 2.000
R-factor	0.2062
Rwork	0.206
R-free	0.21510
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.657
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1-21.2-5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.980	2.072
High resolution limit [Å]	2.000	2.000
Rmerge	0.091
Rpim	0.031
Number of reflections	92614	4365
<I/σ(I)>	21.6
Completeness [%]	97.2	78.83
Redundancy	3
CC(1/2)	0.996	0.791

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	0.2 M ammonium nitrate, 20% w/v PEG3350