9MDB

Co-MAHF-9 A8V Metal Alpha-Helix Framework

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX IV BEAMLINE BioMAX
Synchrotron site	MAX IV
Beamline	BioMAX
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-08-30
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.688801
Spacegroup name	C 2 2 21
Unit cell lengths	10.793, 31.072, 37.915
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.540 - 0.810
R-factor	0.1438
Rwork	0.142
R-free	0.15460
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.686
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.540	0.839
High resolution limit [Å]	0.810	0.810
Number of reflections	6821	657
<I/σ(I)>	18.26
Completeness [%]	99.6
Redundancy	11.7
CC(1/2)	0.998	0.864

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SLOW COOLING		298	water, acetonitrile, cobalt acetate, sodium hydroxide