9MCY

CRYSTAL STRUCTURE OF HUMAN IGG1 FC FRAGMENT-FC-GAMMA RECEPTOR IIA COMPLEX R131 VARIANT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-08-02
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97946
Spacegroup name	P 1
Unit cell lengths	48.993, 76.229, 120.046
Unit cell angles	96.60, 100.24, 90.08

Refinement procedure

Resolution	39.890 - 2.850
R-factor	0.1796
Rwork	0.177
R-free	0.23610
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.182
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.850	2.850
Rmerge	0.179
Rpim	0.112	0.478
Number of reflections	38384	1406
<I/σ(I)>	12.5	1.5
Completeness [%]	95.6	70.1
Redundancy	3.2	2.1
CC(1/2)	0.760	0.640

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	294	10% PEG 5000 MME, 12% isopropanol , 0.1 M MES pH 6.5