9MCX

CRYSTAL STRUCTURE OF HUMAN IGG1 FC FRAGMENT-FC-GAMMA RECEPTOR IIA COMPLEX H131 VARIANT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-06-15
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97946
Spacegroup name	C 1 2 1
Unit cell lengths	161.122, 66.104, 72.094
Unit cell angles	90.00, 94.32, 90.00

Refinement procedure

Resolution	28.020 - 2.380
R-factor	0.187
Rwork	0.184
R-free	0.23410
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.812
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.420
High resolution limit [Å]	2.380	2.380
Rmerge	0.068	0.219
Rpim	0.042	0.139
Number of reflections	28466	1413
<I/σ(I)>	43.9	5.9
Completeness [%]	93.5	93.5
Redundancy	3.5	3.2
CC(1/2)	0.990	0.950

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	294	25% PEG 2000 MME, 0.1 M HEPES pH 7.5