9MBI

8-Oxo-dGTP hydrolysis in human MTH1(G2K mutant) crystal using Mn2+: the ES/EP-3M complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NE3A
Synchrotron site	Photon Factory
Beamline	AR-NE3A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-22
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	46.497, 47.637, 123.839
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.130 - 1.090
R-factor	0.1411
Rwork	0.140
R-free	0.16440
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.007
RMSD bond angle	1.381
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.130	1.110
High resolution limit [Å]	1.090	1.090
Rmerge	0.061	0.552
Number of reflections	115495	5712
<I/σ(I)>	30.7
Completeness [%]	99.9
Redundancy	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	sodium citrate, cacodylate, NaCl