9MAQ
Glycogen phosphorylase from D. longicatena
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-01-28 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 103.969, 122.851, 136.004 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.560 - 3.800 |
| R-factor | 0.2046 |
| Rwork | 0.201 |
| R-free | 0.26550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.678 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.290 | 4.250 |
| High resolution limit [Å] | 3.800 | 3.800 |
| Rmerge | 0.249 | 0.635 |
| Rmeas | 0.270 | 0.687 |
| Rpim | 0.104 | 0.262 |
| Number of reflections | 17775 | 4958 |
| <I/σ(I)> | 10.1 | 5 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.7 | 6.8 |
| CC(1/2) | 0.993 | 0.939 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.2 M Potassium thiocyanate, 28 % PEG 3350 |
