9MAP

Crystal structure of GAGWLP and PD-L1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	193.15
Detector technology	PIXEL
Collection date	2024-10-07
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97861
Spacegroup name	I 41 3 2
Unit cell lengths	222.605, 222.605, 222.605
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.780 - 3.000
R-factor	0.2741
Rwork	0.273
R-free	0.28430
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.544
Data reduction software	autoPROC
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.780	3.080
High resolution limit [Å]	3.000	3.000
Number of reflections	35632	2531
<I/σ(I)>	24.35
Completeness [%]	99.9
Redundancy	400.3
CC(1/2)	1.000	0.777

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289.15	pH 6.0,2.04M NaH2PO4 and K2HPO4