9MAM

The complex structure of Plpro and Frag676

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-07-22
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9793
Spacegroup name	P 32 2 1
Unit cell lengths	81.860, 81.860, 134.900
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.380 - 1.810
R-factor	0.1831
Rwork	0.182
R-free	0.20430
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.942
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.19_4092: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.380	1.860
High resolution limit [Å]	1.810	1.810
Rmerge	0.209	1.746
Rmeas	0.215	1.791
Rpim	0.048	0.397
Total number of observations	957663	70915
Number of reflections	48351	3529
<I/σ(I)>	12.4	2.3
Completeness [%]	99.9
Redundancy	19.8	20.1
CC(1/2)	0.998	0.557

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6	277	1.6 M Sodium Malonate Dibasic Monohydrate