9M80
cryo-EM structure of maltose and glucose bound rice PHS1(R709A)-DPE1(D391A) complex
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
| Staining material | Uranyl Acetate |
EM Data Collection
| Microscope | FEI POLARA 300 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.07 |
| Number of Particle | 2236025 |
| Software | cryoSPARC,PHENIX |
| Symmetry | C1 |
| CTF correction | PHASE FLIPPING ONLY |
