9M6E
Crystal structure of xanthine/guanine RNA aptamer (P1-12bp) with hypoxanthine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-09-12 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.97895 |
| Spacegroup name | P 1 |
| Unit cell lengths | 22.766, 44.905, 209.564 |
| Unit cell angles | 89.91, 89.90, 89.95 |
Refinement procedure
| Resolution | 34.930 - 2.950 |
| R-factor | 0.2474 |
| Rwork | 0.246 |
| R-free | 0.27150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.644 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 104.780 | 3.110 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.156 | 0.646 |
| Rpim | 0.156 | 0.646 |
| Number of reflections | 17227 | 2447 |
| <I/σ(I)> | 4.1 | 1.7 |
| Completeness [%] | 98.4 | 96.6 |
| Redundancy | 3 | |
| CC(1/2) | 0.969 | 0.914 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 0.08 M Potassium chloride 0.02 M Magnesium chloride hexahydrate 48% v/v (+/-)-2-Methyl-2,4-pentanediol 0.012 M Spermine tetrahydrochloride |
