9M6B
Crystal structure of xanthine/guanine RNA (P1-12bp) aptamer with guanine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-10-22 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 |
| Unit cell lengths | 22.885, 46.687, 99.999 |
| Unit cell angles | 95.27, 95.47, 90.55 |
Refinement procedure
| Resolution | 24.780 - 2.610 |
| R-factor | 0.2388 |
| Rwork | 0.236 |
| R-free | 0.30410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.655 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.080 | 2.690 |
| High resolution limit [Å] | 2.610 | 2.620 |
| Rmerge | 0.166 | 0.907 |
| Rpim | 0.166 | 0.907 |
| Number of reflections | 12213 | 858 |
| <I/σ(I)> | 6.4 | |
| Completeness [%] | 98.7 | 97.4 |
| Redundancy | 3.3 | |
| CC(1/2) | 0.951 | 0.288 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 0.08 M Potassium chloride 0.02 M Magnesium chloride hexahydrate 50% v/v (+/-)-2-Methyl-2,4-pentanediol 0.012 M Spermine tetrahydrochloride |
