9M4Z
Cubic insulin crystal, Esrapid, at pH 4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-11-06 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.9 |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 78.010, 78.010, 78.010 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.500 - 1.500 |
| R-factor | 0.197 |
| Rwork | 0.195 |
| R-free | 0.21680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.953 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.554 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 196717 | 12802 |
| <I/σ(I)> | 29.05 | |
| Completeness [%] | 99.9 | |
| Redundancy | 2 | |
| CC(1/2) | 1.000 | 0.565 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20mM citric acid, 0.5M sodium phosphate dibasic dihydrate, 0.1M sodium sulfate, pH 4 |
