9M4S

crystal structure of Arabidopsis thaliana ING2 PHD finger in complex with an H3K4me3 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-01-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	P 61
Unit cell lengths	50.699, 50.699, 55.239
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.907 - 1.601
R-factor	0.187
Rwork	0.185
R-free	0.22460
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	1.501
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.660
High resolution limit [Å]	1.600	3.450	1.600
Rmerge	0.093	0.065	0.339
Rmeas		0.069	0.361
Rpim		0.024	0.123
Number of reflections	10759	1099	1055
<I/σ(I)>	8.6
Completeness [%]	99.8	99.1	100
Redundancy	8.4	8.3	8.6
CC(1/2)	0.946	0.996	0.946

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	0.1M NiCl2, 20% PEG2000 MME, and 0.1M Tris, pH 8.5