9M3N
Crystal structure of human TRIM21 PRYSPRY in complex with T-02
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2023-04-23 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979183 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 100.040, 100.040, 50.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.633 - 2.080 |
| Rwork | 0.191 |
| R-free | 0.23510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.646 |
| Data reduction software | xia2 |
| Data scaling software | Aimless (0.7.7) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.633 | 2.140 |
| High resolution limit [Å] | 2.080 | 2.080 |
| Rmerge | 0.017 | 0.200 |
| Number of reflections | 16022 | 1158 |
| <I/σ(I)> | 22.3 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 1.8 | 1.5 |
| CC(1/2) | 1.000 | 0.955 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | The solution contained 0.1 M BIS-TRIS propane (pH 7.0) and 1.2 M potassium sodium tartrate tetrahydrate. |
