9M20
GmMAN19-1 from Glycine max
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-04-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0903 |
| Spacegroup name | P 63 |
| Unit cell lengths | 84.442, 84.442, 87.182 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 23.938 - 1.389 |
| R-factor | 0.137 |
| Rwork | 0.128 |
| R-free | 0.15240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.858 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.938 | 1.440 |
| High resolution limit [Å] | 1.389 | 1.389 |
| Rmerge | 0.060 | 0.491 |
| Number of reflections | 108306 | 7047 |
| <I/σ(I)> | 28.56 | |
| Completeness [%] | 99.7 | |
| Redundancy | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 283 | 0.2 M Ammonium acetate, 0.1 M Sodium acetate trihydrate pH 4.6 and 30% PEG 4000 |
