9M0K

Crystal structure of the WRKY DNA-binding domain in complex with the W-box DNA motif

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.281490
Spacegroup name	C 2 2 21
Unit cell lengths	54.111, 93.285, 83.694
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.810 - 1.800
R-factor	0.247
Rwork	0.247
R-free	0.28030
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond angle	22.860
Data reduction software	XDS
Data scaling software	XDS ((1.20.1_4487: ???))
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.810	1.840
High resolution limit [Å]	1.800	1.800
Rmerge	0.039	0.219
Number of reflections	19996	1105
<I/σ(I)>	20.4
Completeness [%]	99.7
Redundancy	6.9
CC(1/2)	0.997	0.895

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		289.15	20% PEG 3350, 0.2 M ammonium formate