9M07

Histidine kinase QseE sensor domain of Salmonella Typhimurium SL1344

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-07
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.987
Spacegroup name	P 41 21 2
Unit cell lengths	55.452, 55.452, 86.115
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.490 - 2.400
R-factor	0.2279
Rwork	0.223
R-free	0.27470
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.583
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.400	2.400
Number of reflections	5687	541
<I/σ(I)>	30
Completeness [%]	99.9
Redundancy	23.5
CC(1/2)	0.992	0.985

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	3.5 M Sodium formate, 0.1 M Tris-HCl pH 8.5