9M06

Outer membrane lipoprotein QseG of Salmonella enterica serovar Typhimurium SL1344

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-04-12
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.987
Spacegroup name	C 1 2 1
Unit cell lengths	90.090, 41.001, 126.658
Unit cell angles	90.00, 103.18, 90.00

Refinement procedure

Resolution	37.140 - 2.100
R-factor	0.212
Rwork	0.210
R-free	0.23400
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.180
High resolution limit [Å]	2.100	2.100
Number of reflections	26629	2506
<I/σ(I)>	13.5
Completeness [%]	99.1
Redundancy	6
CC(1/2)	0.977	0.754

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	15% (w/v) PEG8000, 0.1 M sodium cacodylate pH 6.7, 0.2 M magnesium acetate