9LZE
Reductive-half reaction intermediate of copper amine oxidase from Arthrobacter globiformis captured by mix-and-inject serial crystallography at 500-ms time delay
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | FREE ELECTRON LASER |
| Source details | SACLA BEAMLINE BL2 |
| Synchrotron site | SACLA |
| Beamline | BL2 |
| Temperature [K] | 299 |
| Detector technology | CCD |
| Collection date | 2020-10-09 |
| Detector | MPCCD |
| Wavelength(s) | 1.24 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 192.670, 64.690, 158.450 |
| Unit cell angles | 90.00, 116.91, 90.00 |
Refinement procedure
| Resolution | 29.800 - 1.730 |
| R-factor | 0.1842 |
| Rwork | 0.183 |
| R-free | 0.20490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.076 |
| Data reduction software | CrystFEL |
| Data scaling software | CrystFEL |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.790 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Number of reflections | 356066 | 35600 |
| <I/σ(I)> | 3.84 | |
| Completeness [%] | 100.0 | |
| Redundancy | 48.3 | |
| CC(1/2) | 0.922 | 0.512 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 289 | 1.05M potassium/sodium tartrate in 25mM HEPES buffer pH 7.4 |
