9LZ5
Reductive-half reaction intermediate of copper amine oxidase from Arthrobacter globiformis captured by mix-and-inject serial crystallography at 300-ms time delay
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | FREE ELECTRON LASER |
| Source details | SACLA BEAMLINE BL2 |
| Synchrotron site | SACLA |
| Beamline | BL2 |
| Temperature [K] | 299 |
| Detector technology | CCD |
| Collection date | 2022-01-28 |
| Detector | MPCCD |
| Wavelength(s) | 1.24 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 192.832, 64.728, 158.520 |
| Unit cell angles | 90.00, 116.92, 90.00 |
Refinement procedure
| Resolution | 29.820 - 1.870 |
| R-factor | 0.1891 |
| Rwork | 0.188 |
| R-free | 0.21260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.830 |
| Data reduction software | CrystFEL |
| Data scaling software | CrystFEL |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.970 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Number of reflections | 282568 | 28295 |
| <I/σ(I)> | 3.89 | |
| Completeness [%] | 100.0 | |
| Redundancy | 84.8 | |
| CC(1/2) | 0.940 | 0.521 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 289 | 1.05M potassium/sodium tartrate in 25mM HEPES buffer pH 7.4 |
