9LVY

hexamer form of insulin detemir at ambient temperature

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU PhotonJet-R
Temperature [K]	293
Detector technology	PIXEL
Collection date	2023-08-14
Detector	RIGAKU HyPix-Arc 100
Wavelength(s)	1.5
Spacegroup name	H 3
Unit cell lengths	81.240, 81.240, 40.322
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.380 - 2.850
R-factor	0.2905
Rwork	0.285
R-free	0.33770
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.349
Data reduction software	CrysalisPro
Data scaling software	CrysalisPro
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.380	2.951
High resolution limit [Å]	2.850	2.850
Number of reflections	2306	202
<I/σ(I)>	7.39
Completeness [%]	98.5
Redundancy	19
CC(1/2)	0.890	0.745

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.2M sodium acetate trihydrate, 0.1M tris hydrochloride pH 9