9LVX

di-hexamer form of insulin detemir at ambient temperature

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU PhotonJet-R
Temperature [K]	293
Detector technology	PIXEL
Collection date	2023-08-14
Detector	RIGAKU HyPix-Arc 150
Wavelength(s)	1.5
Spacegroup name	H 3
Unit cell lengths	80.898, 80.898, 80.414
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.320 - 2.700
R-factor	0.2778
Rwork	0.273
R-free	0.32230
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.396
Data reduction software	CrysalisPro
Data scaling software	CrysalisPro
Phasing software	PHENIX
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.320	2.797
High resolution limit [Å]	2.700	2.700
Number of reflections	5361	479
<I/σ(I)>	5.81
Completeness [%]	97.3
Redundancy	12.9
CC(1/2)	0.884	0.737

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.2M sodium acetate trihydrate, 0.1M tris hydrochloride pH 9