9LVD

Temperature induces a shift from the dihexamer to the hexamer form of insulin (200K)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU PhotonJet-R
Temperature [K]	200
Detector technology	PIXEL
Collection date	2024-09-17
Detector	RIGAKU HyPix-Arc 150
Wavelength(s)	1.5
Spacegroup name	H 3
Unit cell lengths	80.711, 80.711, 39.640
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	17.550 - 2.850
R-factor	0.3
Rwork	0.280
R-free	0.33000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.432
Data reduction software	HKL-2000
Data scaling software	CrysalisPro
Phasing software	PHENIX
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	17.560	2.951
High resolution limit [Å]	2.850	2.850
Number of reflections	2306	202
<I/σ(I)>	6.46
Completeness [%]	98.5
Redundancy	20
CC(1/2)	0.934	0.330

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2M sodium acetate trihydrate, 0.1M tris hydrochloride pH 9