9LVC
Temperature induces a shift from the dihexamer to the hexamer form of insulin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU PhotonJet-R |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-09-17 |
| Detector | RIGAKU HyPix-Arc 150 |
| Wavelength(s) | 1.5 |
| Spacegroup name | H 3 |
| Unit cell lengths | 79.064, 79.064, 79.496 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.770 - 2.300 |
| R-factor | 0.2375 |
| Rwork | 0.232 |
| R-free | 0.28680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.381 |
| Data reduction software | HKL-2000 |
| Data scaling software | CrysalisPro |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.770 | 2.382 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 8131 | 8131 |
| <I/σ(I)> | 30.01 | |
| Completeness [%] | 98.8 | |
| Redundancy | 4.1 | |
| CC(1/2) | 0.999 | 0.990 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M sodium acetate trihydrate, 0.1M tris hydrochloride pH 9 |
