9LV1
Crystal structure of H1 Haemagglutinin HN/18 from Influenza A virus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2021-05-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 102.339, 79.603, 121.806 |
| Unit cell angles | 90.00, 95.34, 90.00 |
Refinement procedure
| Resolution | 50.010 - 2.400 |
| R-factor | 0.26344 |
| Rwork | 0.263 |
| R-free | 0.28012 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lxv |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX (Phenix1.20.1-4487) |
| Refinement software | REFMAC (5.8.0218) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 121.280 | 2.380 |
| High resolution limit [Å] | 2.320 | 2.320 |
| Rmerge | 0.186 | 0.442 |
| Number of reflections | 65967 | 3023 |
| <I/σ(I)> | 8.3 | |
| Completeness [%] | 89.7 | |
| Redundancy | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2 M Sodium fluoride, 20% w/v Polyethylene glycol 3,350 |
