9LUD
Crystal structure of 9-mer peptide from H5N1 avian influenza virus in complex with B4 DUCK MHC I (UAA*76)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-05-30 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979230 |
| Spacegroup name | P 62 |
| Unit cell lengths | 82.094, 82.094, 111.370 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.839 - 2.501 |
| Rwork | 0.176 |
| R-free | 0.24690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.401 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0415) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.000 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.069 | 0.166 |
| Number of reflections | 14802 | 1578 |
| <I/σ(I)> | 22.9 | |
| Completeness [%] | 99.2 | |
| Redundancy | 9.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | Salt: none Buffer: 0.04 M Citric acid, 0.06 M BIS-TRIS propane / pH 6.4 Polymer: 20% w/v Polyethylene glycol 3,350 |
