9LU3

LN1F9 Fab bound to Nipah Virus attachment (G) glycoprotein head domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-11-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	123.416, 125.155, 152.376
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.440 - 3.000
R-factor	0.2219
Rwork	0.219
R-free	0.26880
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.326
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.600	3.100
High resolution limit [Å]	3.000	3.000
Rmerge	0.128	0.594
Number of reflections	47546	4516
<I/σ(I)>	5.6	1.8
Completeness [%]	99.1
Redundancy	5.2
CC(1/2)	0.983	0.754

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		289	0.3 M ammonium formate, 0.1 M HEPES pH 7.0, 20 % [vol/vol] Sokalan CP5