9LTY
Crystal Structure of C-terminal stable fragment of the Shaft pilin EbpC from Enterococcus faecalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 11.2C |
| Synchrotron site | ELETTRA |
| Beamline | 11.2C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-11-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 33.542, 47.600, 125.682 |
| Unit cell angles | 91.68, 90.18, 89.87 |
Refinement procedure
| Resolution | 125.630 - 2.000 |
| R-factor | 0.22468 |
| Rwork | 0.223 |
| R-free | 0.24839 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.469 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 125.630 | 2.030 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmerge | 0.067 | 0.466 |
| Rmeas | 0.080 | 0.567 |
| Rpim | 0.043 | 0.317 |
| Number of reflections | 50621 | 2139 |
| <I/σ(I)> | 9.2 | 1.8 |
| Completeness [%] | 96.0 | |
| Redundancy | 3.2 | |
| CC(1/2) | 0.996 | 0.817 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 200mM Magnesium Chloride, 100mM Tris- HCl pH 8.5, 25% (w/v) PEG 3350 |
