9LTQ

Crystal structure of human RAB43 in complex with GppNHp

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-12-11
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97851
Spacegroup name	C 1 2 1
Unit cell lengths	89.780, 77.858, 64.064
Unit cell angles	90.00, 128.41, 90.00

Refinement procedure

Resolution	27.480 - 1.600
R-factor	0.1682
Rwork	0.166
R-free	0.20400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.884
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX (1.21.1_5286)
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.650
High resolution limit [Å]	1.600	1.600
Rmerge	0.097	0.177
Number of reflections	44897	4388
<I/σ(I)>	8.8	3.9
Completeness [%]	97.7
Redundancy	3.4	3.2
CC(1/2)	0.987	0.963

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	0.1M Imidazole/MES monohydrate, pH6.5; 25% MPD, 25% PEG 1000, 25% PEG 3350; 0.02M DL-Glutamic acid monohydrate, 0.02M DL-Alanine, 0.02M Glycine, 0.02M DL-Lysine monohydrochloride, 0.02M DL-Serine