9LT9

Crystal structure of Gle1 from Debaryomyces hansenii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2022-10-07
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.987
Spacegroup name	P 21 21 21
Unit cell lengths	47.222, 70.697, 87.929
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.850 - 1.500
R-factor	0.1725
Rwork	0.172
R-free	0.19340
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.976
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.550
High resolution limit [Å]	1.500	1.500
Number of reflections	47692	4729
<I/σ(I)>	49.5
Completeness [%]	99.3
Redundancy	10.4
CC(1/2)	0.993	0.927

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		280	Tris (pH 8.5) and 25% (w/v) PEG 3350