9LSN
hAGO2-MID in complex with a chemical modified uridine monophosphate
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-12-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 |
| Unit cell lengths | 40.480, 46.381, 66.472 |
| Unit cell angles | 86.86, 73.99, 85.09 |
Refinement procedure
| Resolution | 31.930 - 1.750 |
| R-factor | 0.1723 |
| Rwork | 0.170 |
| R-free | 0.21760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.027 |
| Data reduction software | XDS |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.18.2_3874: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.190 | 1.840 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 88356 | 44945 |
| <I/σ(I)> | 4.6 | |
| Completeness [%] | 96.3 | |
| Redundancy | 1.8 | |
| CC(1/2) | 0.996 | 0.791 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 290 | 0.25M Ammonium sulfate 0.1M Sodium cacodylate trihydrate pH 6.5 30% w/v Polyethylene glycol 8,000 |
