9LS8
Crystal structure of peptidyl-tRNA hydrolase from Enterococcus faecium at 1.22 A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-09-06 |
| Detector | DECTRIS EIGER2 X CdTe 16M |
| Wavelength(s) | 0.885 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.765, 47.584, 69.168 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.060 - 1.220 |
| R-factor | 0.16019 |
| Rwork | 0.158 |
| R-free | 0.19805 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.657 |
| Data reduction software | XDS |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.580 | 1.240 |
| High resolution limit [Å] | 1.220 | 1.220 |
| Number of reflections | 40928 | 2262 |
| <I/σ(I)> | 13.4 | |
| Completeness [%] | 88.7 | |
| Redundancy | 4.3 | |
| CC(1/2) | 0.997 | 0.710 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 0.2M Ammonium Sulphate, 0.1M Tris-HCl pH 7.5, 15% PEG 4000 |
