9LS7
The crystal structure of human RyR3 Repeat12 domain complex with RI-2 and AMP-PCP
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 297 |
| Detector technology | PIXEL |
| Collection date | 2025-04-30 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.356, 61.617, 277.585 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 16.960 - 2.490 |
| Rwork | 0.234 |
| R-free | 0.27100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | HKL-2000 |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.14) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 138.790 | 2.660 |
| High resolution limit [Å] | 2.490 | 2.490 |
| Rmerge | 0.164 | 0.952 |
| Rmeas | 0.174 | |
| Number of reflections | 29417 | 1471 |
| <I/σ(I)> | 8.2 | 1.4 |
| Completeness [%] | 92.1 | |
| Redundancy | 8.3 | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291.5 | 0.1M Tris-HCl pH 8.5, 20% (w/v) PEG 1000, 10% Glycerol |
