9LQG
Crystal structure of SAM lyase in complex with MTA-AMP
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 153 |
| Detector technology | PIXEL |
| Collection date | 2024-04-20 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 94.653, 54.424, 70.272 |
| Unit cell angles | 90.00, 95.03, 90.00 |
Refinement procedure
| Resolution | 47.140 - 1.280 |
| R-factor | 0.1938 |
| Rwork | 0.193 |
| R-free | 0.20890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.042 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.21.1_5286: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.090 | 1.310 |
| High resolution limit [Å] | 1.280 | 1.280 |
| Number of reflections | 91712 | 6729 |
| <I/σ(I)> | 8.6 | |
| Completeness [%] | 100.0 | |
| Redundancy | 6.3 | |
| CC(1/2) | 0.991 | 0.203 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2 M Ammonium sulfate, 20% w/v Polyethylene glycol 3,350 |
