9LQB

Crystal structure of NrN E21A in complex with SAH-AMP

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	153
Detector technology	PIXEL
Collection date	2024-04-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 41 21 2
Unit cell lengths	71.270, 71.270, 121.530
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.220 - 1.930
R-factor	0.2422
Rwork	0.240
R-free	0.28300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.658
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.21.1_5286: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.220	1.980
High resolution limit [Å]	1.930	1.930
Number of reflections	24067	1693
<I/σ(I)>	19.9
Completeness [%]	99.3
Redundancy	21.2
CC(1/2)	0.997	0.371

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	277	0.1 M Succinic acid pH 7.0, 12% w/v Polyethylene glycol 3,350