9LP4

Structural insights into the Tumor suppressor ZMYND11 reveal diverse recognition mechanisms

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	198
Detector technology	PIXEL
Collection date	2024-09-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	40.136, 73.848, 116.695
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.920 - 2.960
R-factor	0.2096
Rwork	0.208
R-free	0.23830
Structure solution method	AB INITIO PHASING
RMSD bond length	0.011
RMSD bond angle	1.812
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	HKL2Map
Refinement software	PHENIX (2.0_5761)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.030
High resolution limit [Å]	2.960	2.980
Number of reflections	7394	7036
<I/σ(I)>	16.6
Completeness [%]	90.1
Redundancy	7.3
CC(1/2)	0.992	0.874

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	289	30% MPD, 10%PEG4000,100 mM Imidazole pH 6.5