9LOW

Crystal structure of RamR3.0 complexed with 3-alkenynaphthoate

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-01-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97915
Spacegroup name	P 1
Unit cell lengths	45.402, 54.793, 84.885
Unit cell angles	98.14, 95.86, 96.91

Refinement procedure

Resolution	28.419 - 2.500
R-factor	0.2519
Rwork	0.250
R-free	0.28730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3vw0
RMSD bond length	0.007
RMSD bond angle	1.019
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX ((1.16_3549: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	83.410	2.600
High resolution limit [Å]	2.500	2.500
Rmerge	0.134	0.717
Rmeas	0.158	0.843
Rpim	0.084
Number of reflections	26685	2979
<I/σ(I)>	6.6	1.4
Completeness [%]	96.7	96.8
Redundancy	3.5	3.6
CC(1/2)	0.992	0.655

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291.15	0.1M MES, pH6.5, 1.6 M MgSO4