9LNR

Crystal structure of SKLB-D18 with ERK2

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-11-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	44.015, 70.482, 120.074
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	60.780 - 2.100
R-factor	0.2135
Rwork	0.210
R-free	0.27700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond angle	16.951
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.21_5207: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.780	2.210
High resolution limit [Å]	2.100	2.100
Number of reflections	22718	3264
<I/σ(I)>	8.3
Completeness [%]	100.0
Redundancy	13.1
CC(1/2)	0.997	0.465

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM MES pH 7.5, 25% PEG MME 2000, 200 mM Ammonium sulfate, 20 mM DTT