9LNP

the hUNG bound to DNA product embedding uridine ribonucleotide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	93
Detector technology	PIXEL
Collection date	2021-07-04
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.979183
Spacegroup name	P 21 21 21
Unit cell lengths	48.930, 65.290, 99.530
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.790 - 1.760
R-factor	0.17
Rwork	0.168
R-free	0.20750
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.940	1.810
High resolution limit [Å]	1.760	1.760
Rmerge	0.186
Number of reflections	414484	2307
<I/σ(I)>	10.26
Completeness [%]	99.7
Redundancy	12.9
CC(1/2)		0.430

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	283	20% polyethylene glycol 4000 (Sigma)/100 mM Hepes buffer (pH 6.5)/10% dioxane/1 mM DTT