9LNF

Crystal structure of SpoIVB_101-426-S378A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-11-03
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 62
Unit cell lengths	72.190, 72.190, 108.440
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	23.630 - 2.490
R-factor	0.2379
Rwork	0.236
R-free	0.28740
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.284
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.590
High resolution limit [Å]	2.490	2.490
Rmerge	0.117
Number of reflections	11228	1143
<I/σ(I)>	32.8
Completeness [%]	100.0
Redundancy	20.2
CC(1/2)	0.995	0.787

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	25% PEG3350, 0.2 M lithium sulfate, and 0.1 M HEPES pH7.5