9LN2
Crystal Structure of Intracellular B30.2 Domain of VpBTN3 in Complex with DMASPP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | LIQUID ANODE |
| Source details | BRUKER METALJET |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-07-29 |
| Detector | Bruker PHOTON III |
| Wavelength(s) | 1.34138 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.858, 67.988, 134.307 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.750 - 1.770 |
| R-factor | 0.15937 |
| Rwork | 0.157 |
| R-free | 0.20163 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.707 |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.750 | 1.800 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Rmerge | 0.046 | 0.168 |
| Number of reflections | 39950 | 1878 |
| <I/σ(I)> | 31.9 | |
| Completeness [%] | 99.6 | |
| Redundancy | 11.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.1 M Bis Tris pH5.5, 2.0 M ammonium sulfate |
