Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2021-08-10 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.201, 123.744, 82.039 |
| Unit cell angles | 90.00, 97.79, 90.00 |
Refinement procedure
| Resolution | 27.110 - 1.800 |
| R-factor | 0.18158 |
| Rwork | 0.180 |
| R-free | 0.21387 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.151 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.740 |
| High resolution limit [Å] | 1.680 | 1.680 |
| Rmerge | 0.051 | 0.748 |
| Rpim | 0.033 | |
| Number of reflections | 86662 | 11486 |
| <I/σ(I)> | 19.94 | |
| Completeness [%] | 98.7 | 99.7 |
| Redundancy | 3.3 | 3.2 |
| CC(1/2) | 0.638 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 300 | 0.1 M HEPES, 25% PEG3350 |
