9LM9

Crystal structure of bvFP-Pfr21

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-11-03
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.987
Spacegroup name	P 21 21 2
Unit cell lengths	159.320, 77.000, 86.410
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.620 - 3.240
R-factor	0.324
Rwork	0.319
R-free	0.36680
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.647
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.620	3.320
High resolution limit [Å]	3.240	3.240
Number of reflections	16978	1277
<I/σ(I)>	7.6
Completeness [%]	96.9
Redundancy	3.2
CC(1/2)	0.996	0.550

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		300	10% (w/v) PEG 8000; 100mM imidazole/Hydrochloric acid pH 8.0